Fourth generation Glypican-3–specific CAR-NK cells co-secreting IL-15 and IFN-α have increased anti-tumor function versus hepatocellular carcinoma

IL-21 in Synergy with IL-15 or IL-18 Enhances IFN- Production in Human NK and T Cells

Glypican-3 as a tumor marker for hepatocellular carcinoma

Article history: Received on: 13/03/2013 Revised on: 04/04/2013 Accepted on: 05/05/2013 Available online: 27/06/2013 Hepatocellular carcinoma(HCC) is one of the most frequent cancers in the world.The burden of HCC has been increasing in Egypt with a doubling in the incidence in the past ten years. The prognosis of most patients is unsatisfactory due to rapid clinical deterioration after the ini...

Anti-GPC3-CAR T Cells Suppress the Growth of Tumor Cells in Patient-Derived Xenografts of Hepatocellular Carcinoma

BACKGROUND The lack of a general clinic-relevant model for human cancer is a major impediment to the acceleration of novel therapeutic approaches for clinical use. We propose to establish and characterize primary human hepatocellular carcinoma (HCC) xenografts that can be used to evaluate the cytotoxicity of adoptive chimeric antigen receptor (CAR) T cells and accelerate the clinical translatio...

Molecular analysis of the methylprednisolone-mediated inhibition of NK-cell function: evidence for different susceptibility of IL-2- versus IL-15-activated NK cells.

Steroids have been shown to inhibit the function of fresh or IL-2-activated natural killer (NK) cells. Since IL-15 plays a key role in NK-cell development and function, we comparatively analyzed the effects of methylprednisolone on IL-2- or IL-15-cultured NK cells. Methylprednisolone inhibited the surface expression of the major activating receptors NKp30 and NKp44 in both conditions, whereas N...

Plasmodium falciparum-Infected Erythrocytes and IL-12/IL-18 Induce Diverse Transcriptomes in Human NK Cells: IFN-α/β Pathway versus TREM Signaling

The protective immunity of natural killer (NK) cells against malarial infections is thought to be due to early production of type II interferon (IFN) and possibly direct NK cell cytotoxicity. To better understand this mechanism, a microarray analysis was conducted on NK cells from healthy donors PBMCs that were co-cultured with P. falciparum 3D7-infected erythrocytes. A very similar pattern of ...